Primer Its1. The ITS1F/ITS4B primer pair produced a small amount of PCR product for certain plant species but the quantity was in most cases less than that produced by the ‘universal’ ITS primers However under conditions where both plant and fungal DNAs were present the fungal DNA was amplified to the apparent exclusion of plant DNA.
Its1 from home.psu.ac.th
The forward primer ITS1OF overlaps with ITS1F but is positioned two bases 5′ in the small subunit Note that ITS1OF is really two primers of nearly identical sequence that must be ordered separately and then combined before use synthesis of a single degenerate primer is not recommended The altered placement and two positions that differ among the primer.
Primers ITS1, ITS2 and ITS4 detect the intraspecies
Primers ITS1 ITS2 and ITS4 detect the intraspecies variability in the internal transcribed spacers and 58S rRNA gene region in clinical isolates of fungi Restriction fragment length polymorphism analysis of the 58S rRNA gene and the internal transcribed spacers (ITS1 and ITS2) was used for examination of 66 isolates belonging to 19 species.
Conserved primer sequences for PCR amplification of fungal
Primers ITS1 CF and ITS1 BR previously designed to amplify the internal transcribed spacer (ITS1) of rDNA have been evaluated for use in a universal diagnostic test for all pathogenic trypanosomes Blood was collected from 373 cattle and 185 camels The primers gave constant PCR products with the stocks of each taxon tested.
Where I can order fungal ITS1 and ITS4 primers?
The ITS1F ( Gardes and Bruns 1993) and ITS4 ( White et al 1990) primers appear to have specificity for the ascomycetous basidiomycetous and zygomycetous fungi ( Gardes and Bruns 1993 Dickie et al 2002 Klamer et al 2002 Anderson et al 2003a Anderson et al 2003b Izzo et al 2005 ).
Its1
Use of the ITS primers, ITS1F and ITS4, to characterize
ITS primers with enhanced specificity for basidiomycetes
Primers Its1 Macrogen Bioz
of … developed for analysis Fungalspecific PCR primers
Internal transcribed spacer primers and sequences for
ITS Illumina Amplicon Protocol : earthmicrobiome
ITS1 versus ITS2 as DNA metabarcodes for fungi
Comparison and Validation Primer Pairs … of Some ITS
Use of the ITS primers, ITS1F and ITS4, to characterize
Wikipedia Internal transcribed spacer
Targeting of Internal Transcribed Spacer Use of PCR
for the DNABased Identification HighCoverage ITS Primers
The primers ITS1f (Gardes & Bruns 1993 ) which targets a site in the ribosomal small subunit (SSU) encoding region and ITS4 (White et al 1990 ) which targets an ITSflanking site in the ribosomal large subunit (LSU) encoding region were devised based on the scarce sequence information that was available at the time.
